Benefits of RNA Sequencing. Download eBook. Access PDF. New To NGS? Find out how Illumina NGS technology works and what types of experiments it enables. Microarrays RNA-Seq vs. Learn More Ribosome Profiling Deeply sequence ribosome-protected mRNA fragments to gain a complete view of the ribosomes active in a cell at a specific time point, and predict protein abundance. Learn More. Frequently Purchased Together. Your items have been added to the cart.
Continue Shopping Go to Cart. Read Customer Interview. The Time Is Now for Microbiome Studies Transcriptomics and whole-genome shotgun sequencing provide researchers and pharmaceutical companies with data to refine drug discovery and development. Read Interview. Read Technical Bulletin. Featured Products. Illumina Stranded mRNA Prep A simple, scalable, cost-effective, rapid single-day solution for analyzing the coding transcriptome leveraging as little as 25 ng input of standard non-degraded RNA.
View Product. Interested in receiving newsletters, case studies, and information on genomic analysis techniques? Enter your email address. Additional Resources. Download Now. Revisiting global gene expression analysis. Qing, T. China Life Sci. Leshkowitz, D. Using synthetic mouse spike-in transcripts to evaluate RNA-seq analysis tools.
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This series will discuss RNA-seq from start to finish though not in comprehensive detail , including how to set up a good RNA-seq experiment and how to deal with the resulting data. For now, though, we will simply discuss why RNA-seq may be the right choice for your next gene expression experiment.
Where DNA is the underlying blueprint for all cellular processes, RNA is the molecule produced on demand when those processes are needed. Proteins translated from messenger RNA then carry out the encoded functions. It can reveal problems in the underlying DNA code, as well as defects in processing machinery that ultimately lead to disregulated gene expression or defective proteins.
For example, where a DNA coding region might look normal, a downstream transcriptional problem could lead to alternative splicing of the resulting RNA molecule, leading in turn to a non-functional enzyme and inducing a disease state. These splice variants might not be pathogenic, either.
In some cases, sequencing the RNA can reveal sequences that produce different protein isoforms. In cancer, kidney disease, cardiovascular conditions, autoimmune disease, and many more, changes in gene expression are the underlying cause.
Historically, researchers have used protein levels as a readout in these instances. RNA-seq holds a number of advantages over protein microarrays and their sister assay, RNA microarray.
First, RNA-seq has a much lower detection threshold than arrays learn more about the differences between the two assays in this blog post. The latter involve hybridization of isolated protein to an assay plate that includes a fluorescent marker. More protein causes a brighter signal. That signal has to be high enough for detection, though, necessitating a certain level of starting material. RNA-seq experiments require as little as picograms of starting material, and there are assays for RNA-seq on single cells.
See the Cofactor website for current offerings and sample submission requirements.
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